Dear Andrew, 

Thank you for the advice. I reviewed my connectivity matrices, and I found one matrix was invalid. 

I ran a correlational analysis in a single group to find the subnetwork reflecting correlational relationship associated with the clinical variable. 
However, I couldn't find any significant result, so I am not sure my design matrix and contrast are correct. 


My design matrix is as follows: 
1 0.6
1 -0.4
1 -1.5
1 0.2
1 -0.7
. .
. .
. .

so on..

which first column indicates global mean and second column indicates the clinical variable. 

I set my contrast as [0 1] for positive correlation, and [0 -1] for negative. 
I ran t-test and set t-threshold ranged from 1.0~3.0. 

Is this design and analysis correct for my hypothesis? 



Sicnerely, 
Jean