NIRS Brain AnalyzIR toolbox is an open-source Matlab-based analysis package for fNIRS data management, pre-processing, and first- and second-level (i.e., single- and group-level) statistical analysis. Developed and maintained by Dr. Ted Huppert's lab at University of Pittsburgh. This toolbox was designed to capture and preserve as much of this fNIRS-specific information and noise as possible through the entire analysis pipeline such that first- and higher-level statistical analysis methods could use this information in statistical models by utilizing covariance whitening, accounting for dependent noise terms, and using robust statistical methods. Furthermore, one of the key features of this toolbox is a framework for performing sensitivity-specificity (ROC analysis) to compare analysis methods and pipelines. The current toolbox interfaces to both the popular HOMER-2 and NIRS-SPM fNIRS analysis packages, which allows a head-to-head comparison of various analysis options.