Hi, I used the Fiji GUI. I registered the image and I have the corresponding output files. I would like to know if I can use these files to apply the same transformation to another stack, and how. Thank you in advance,

Andrea

Hi Andrea -

Yes, you should be able to apply the same transformations to different image stacks.

Look for the "reformatx" binary. The general command line syntax is like so:

  reformatx -o OUT --floating FLT REF XFORMS

where

OUT is the path/filename of the output image, FLT is the floating (or moving) image, and REF is the reference (or fixed) image. Basically, the floating image is resliced to the space of the reference image (you can configure what type of interpolation is used in the process; see output of "reformatx --help" for details.

Further, XFORMS is a series of one or more transformations, which are applied in concatentation. Each of the transformations can be prefixed with "-i" (or "--inverse", if you prefer), to apply the inverse transform instead. Note that only linear (affine or rigid) transformations can be exactly inverted. For nonrigid (warp) transformations, the inverse is approximated numerically, which takes a lot more time and may fail if the transformation is non-invertible or otherwise poorly behaved.

As a specific example, note that if

  reformatx --floating A B X1 X2 X3

reslices B to A, then the following will reslice A to B:

  reformatx --floating B A -i X3 -i X2 -i X1

That is, to invert a series of transformations, you'd have to invert each one *and* reverse the order of transformations. (I suspect that's way more than you needed to know).

Hope this helps! As for where Fiji actually has CMTK store the transformations you need, and how they are named, that would need to be answered by someone else.

Best,
Torsten

Originally posted by Torsten Rohlfing:
Just to add that if you were using the Fiji CMTK registration GUI your output registrations will be stored in a folder like so:


if the root folder of your registration project is called myregfolder.