Hi all-

I am learning fMRI analyses, starting with a resting state data set.  We have patients and controls and would like to characterize differences in connectivity between a seed in bilateral MFG using the conn toolbox 15.f.  The data has already been preprocessed - slice time corrected, realigned, coregistered, segmented, normalized and smoothed.  
My initial questions are:
1- Can we skip preprocessing in this version or would you recommend preprocessing within conn?
2- How do I define the MFG seed - simply select bilateral MFG and delete other ROIs from the selected atlasROI.nii or manually define MFG across participants?


Thanks so much for any feedback, 
Best,
-Denise