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users > Rigid registration weird test result
Aug 9, 2018 03:08 AM | Zhilei Zhao - Princeton University
Rigid registration weird test result
Dear CMTK users,
My name is Zhilei. I'm trying to register some confocal images of antennal lobe. Since I just started to learn CMTK, the first thing I did is to run a test registration: put one confocal stacks in the refbrain folder, then use the Fiji to translate 20 pixels in X and Y, then register this transformed stacks with the following command:
registrationx -v --dofs 3 -o Registration/affine/OrcoA11_OrcoAfake_01_9dof.list --write-reformatted temp.nrrd refbrain/OrcoA11_01.nrrd images/OrcoAfake_01.nrrd
If everything works, I should get transformation only in X and Y. However, the transformation result seems unexpected:
Resulting transformation parameters:
#0: 7.400000
#1: 0.300000
#2: 0.400000
#3: 0.000000
#4: 0.000000
#5: 0.000000
#6: 1.000000
#7: 1.000000
#8: 1.000000
#9: 0.000000
#10: 0.000000
#11: 0.000000
#12: 53.305594
#13: 53.305594
#14: 34.997497
When I check the reformatted image, it seems rotated 180 degree. I attached a screenshot showing a stack of the ref image, float image and reformatted image at the same z depth.
I feel I may get some fundamental things wrong. Your help is very appreciated!
My name is Zhilei. I'm trying to register some confocal images of antennal lobe. Since I just started to learn CMTK, the first thing I did is to run a test registration: put one confocal stacks in the refbrain folder, then use the Fiji to translate 20 pixels in X and Y, then register this transformed stacks with the following command:
registrationx -v --dofs 3 -o Registration/affine/OrcoA11_OrcoAfake_01_9dof.list --write-reformatted temp.nrrd refbrain/OrcoA11_01.nrrd images/OrcoAfake_01.nrrd
If everything works, I should get transformation only in X and Y. However, the transformation result seems unexpected:
Resulting transformation parameters:
#0: 7.400000
#1: 0.300000
#2: 0.400000
#3: 0.000000
#4: 0.000000
#5: 0.000000
#6: 1.000000
#7: 1.000000
#8: 1.000000
#9: 0.000000
#10: 0.000000
#11: 0.000000
#12: 53.305594
#13: 53.305594
#14: 34.997497
When I check the reformatted image, it seems rotated 180 degree. I attached a screenshot showing a stack of the ref image, float image and reformatted image at the same z depth.
I feel I may get some fundamental things wrong. Your help is very appreciated!
Threaded View
Title | Author | Date |
---|---|---|
Zhilei Zhao | Aug 9, 2018 | |
Greg Jefferis | Aug 9, 2018 | |
Zhilei Zhao | Aug 9, 2018 | |
Greg Jefferis | Aug 9, 2018 | |
Zhilei Zhao | Aug 10, 2018 | |