users > Rigid registration weird test result
Aug 9, 2018  03:08 AM | Zhilei Zhao - Princeton University
Rigid registration weird test result
Dear CMTK users,

My name is Zhilei. I'm trying to register some confocal images of antennal lobe. Since I just started to learn CMTK, the first thing I did is to run a test registration: put one confocal stacks in the refbrain folder, then use the Fiji to translate 20 pixels in X and Y, then register this transformed stacks with the following command:
registrationx -v --dofs 3 -o Registration/affine/OrcoA11_OrcoAfake_01_9dof.list --write-reformatted temp.nrrd refbrain/OrcoA11_01.nrrd images/OrcoAfake_01.nrrd

If everything works, I should get transformation only in X and Y. However, the transformation result seems unexpected:
Resulting transformation parameters:
#0: 7.400000
#1: 0.300000
#2: 0.400000
#3: 0.000000
#4: 0.000000
#5: 0.000000
#6: 1.000000
#7: 1.000000
#8: 1.000000
#9: 0.000000
#10: 0.000000
#11: 0.000000
#12: 53.305594
#13: 53.305594
#14: 34.997497

When I check the reformatted image, it seems rotated 180 degree. I attached a screenshot showing a stack of the ref image, float image and reformatted image at the same z depth. 

I feel I may get some fundamental things wrong. Your help is very appreciated!
Attachment: CMTK_test.png

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TitleAuthorDate
Rigid registration weird test result
Zhilei Zhao Aug 9, 2018
Greg Jefferis Aug 9, 2018
Zhilei Zhao Aug 9, 2018
Greg Jefferis Aug 9, 2018
Zhilei Zhao Aug 10, 2018