users > RE: File format and registration problem
Mar 25, 2011  12:03 PM | Thomas Templier
RE: File format and registration problem
Thank you Torsten and Greg.
When I reopen the .nrrd file with Fiji and check the properties I have this :

NRRD0004
# Created by Nrrd_Writer at Wed Mar 23 16:50:58 CET 2011
type: uint16
encoding: raw
endian: big
dimension: 3
sizes: 1024 640 119
space dimension: 3
space directions: (0.013888888888888888,0,0) (0,0.013888888888888888,0) (0,0,1.0)
space units: "cm" "cm" "cm"
------------------------
Title: flt.nrrd
Width: 14.22 cm (1024)
Height: 8.89 cm (640)
Depth: 119.00 cm (119)
Resolution: 72 pixels per cm
Voxel size: 0.01x0.01x1 cm
ID: -2
Coordinate origin: 0,0,0
Bits per pixel: 16 (unsigned)
Display range: 88 - 467
Image: 1/119
No Threshold
Uncalibrated
Path: P:\FirstConfoca1403l\flt.nrrd
No Selection

So I don't know exactly what you meant with "sensible properties" (voxel size), but it seems to be defined.
Using the command with -v like Torsten proposed, I have roughly :

... ref.nrrd
Read 1024x512x102 voxels [14.208333 x 7.091222 x 101.0000 mm total size].
Date type ushort <16bit unsigne>, range [61 .. 4095]
WARNING: image does not have valid orientation meta information;cannot reorient.
Read 1024x640x119 voxels [14.208333 x 8.875 x 118.0000 mm total size].
Date type ushort <16bit unsigne>, range [83 .. 4095]
WARNING: image does not have valid orientation meta information;cannot reorient.
Entering resolution level 1 out of 5...
Entering resolution level 1 out of 5...
Entering resolution level 2 out of 5...
Entering resolution level 2 out of 5...
Entering resolution level 3 out of 5...
Entering resolution level 3 out of 5...
Entering resolution level 4 out of 5...
Entering resolution level 4 out of 5...
Entering resolution level 5 out of 5...
Entering resolution level 5 out of 5...
Resulting transformation parameters:
#0: 0.0
#1: -0.88889
#2: -8.5
#3:-0
#4:-0
#5:-0
#6:1.0
#7:1.0
#8:.10
#9:0.0
#10:0.0
#11:0.0
#12:7.104
#13:4.4375
#14: 59
#

And I have no affine.xform file or directory anywhere. (it should be in the same directory as where registration.exe is or in the directory of my images ? I also tried
-o C:\Users\templier\Desktop\affine.xform
and nothing appears)

(By the way, I struggle to have this output automatically in an output file of in my clipboard and it does not work, using different windows command :
registration --initxlate --dofs 6,9 --auto-multi-levels 4 -v -o affine.xform C:\CMTK_TEST\ref.nrrd C:\CMTK_TEST\flt.nrrd > C:\Users\templier\Desktop\dir.txt
registration --initxlate --dofs 6,9 --auto-multi-levels 4 -v -o affine.xform C:\CMTK_TEST\ref.nrrd C:\CMTK_TEST\flt.nrrd | clip )
I also noticed that after one or two computations (I run it first, then a bit later the same task) the program crashes : windows says "registration.exe has stopped working" and in the command line I have

... ref.nrrd

Read 1024x512x102 voxels [14.208333 x 7.091222 x 101.0000 mm total size].

Date type ushort <16bit unsigne>, range [61 .. 4095]

WARNING: image does not have valid orientation meta information;cannot reorient.Read 1024x640x119 voxels [14.208333 x 8.875 x 118.0000 mm total size].


Date type ushort <16bit unsigne>, range [83 .. 4095]


WARNING: image does not have valid orientation meta information;cannot reorient.

Entering resolution level 1 out of 5...
Multi-level R

For my next scans, I´ll use the format directly given by my microscope (olympus > oib), and see what it gives.

Greg, I'll take a closer look at what you have done for fly brains registration, that's what I would like to do.#

Thank you for your help

Thomas

Threaded View

TitleAuthorDate
Thomas Templier Mar 23, 2011
Torsten Rohlfing Mar 28, 2011
Thomas Templier Mar 30, 2011
Torsten Rohlfing Mar 30, 2011
Thomas Templier Apr 4, 2011
Torsten Rohlfing Apr 4, 2011
Thomas Templier Apr 4, 2011
Thomas Templier May 4, 2011
Greg Jefferis May 4, 2011
Torsten Rohlfing Mar 23, 2011
Thomas Templier Mar 24, 2011
Torsten Rohlfing Mar 24, 2011
RE: File format and registration problem
Thomas Templier Mar 25, 2011
Torsten Rohlfing Mar 25, 2011
Thomas Templier Mar 27, 2011
Torsten Rohlfing Mar 27, 2011
Torsten Rohlfing Mar 25, 2011
Greg Jefferis Mar 25, 2011
Greg Jefferis Mar 24, 2011
Torsten Rohlfing Mar 23, 2011