Hello Torsten,

I tested it with two raw datas of confocal images of whole fly brains. Fly brains were stained with phaloidine rodamine.

I've used the command

Then, to see the result I've used

The resulting reformat.nrrd is shifted on the top, as if the transformation was a translation in direction of the top (with amplitude = half of the image height). That is why I say it failed.

With an other image pair, the translation is extreme on the left, and a very little part of the image can be seen.

Maybe I missed some preprocessing steps. Let me know if you need some more information.

Best Regards,

Thomas